Pandurang Kolekar

Pan-cancer Detection of Solid Tumor Mutations in Cell-free DNA

​

In this study, we have assessed tumor-plasma concordance of mutations in >120 patients across 9 different cancer types where 50% of the samples were characterized as Stages I and II. To address issues of sensitivity of detection, we have utilized two types of digital technologies - ddPCR and targeted NGS. We demonstrate the clinical utility of detecting low levels of ctDNA in various tissues types and stages of cancer.

​

Developed data analysis pipelines for molecularly barcoded NGS data for cfDNA from liquid biopsy samples.

 

Publication:

​

Balaji SA, Shanmugam A, Chougule A, Sridharan S, Prabhash K, Arya A, Chaubey A, Hariharan A, Kolekar P, Sen M, Ravichandran A, Katragadda S, Sankaran S, Bhargava S, Kulkarni P4, Rao S, Sunkavalli C, Banavali S, Joshi A, Noronha V, Dutt A, Bahadur U, Hariharan R, Veeramachaneni V, Gupta V. (2018)

Analysis of solid tumor mutation profiles in liquid biopsy. Cancer Medicine, 00:1–9. https://doi.org/10.1002/cam4.1791 PMID: 30264478

​

​